What are Lentiviral Vectors

Lentiviral vectors (LV) are viral-based gene delivery systems that can stably deliver genes or RNAi into primary cells or cell lines with up to 100% efficiency. LVs bind to target cells using an envelope protein which allows for release of the LV RNA containing the gene or gene silencing sequence into the cell. The LVs RNA is then converted into DNA using an enzyme called reverse transcriptase by a process called reverse transcription. The DNA pre-integration complex then enters the nucleus and integrates into the target cell's chromosomal DNA. Gene delivery is stable because the target gene or gene silencing sequence is integrated in the chromosome and is copied along with the DNA of the cell every time the cell divides. One of the discriminating features of LVs is their ability to integrate into non-dividing cells, in contrast to other vectors that either don't integrate efficiently into chromosomal DNA (e.g. non-viral, Adenoviral and Adenoviral-Associated vectors) or can only integrate upon cell division (e.g. conventional Retroviral vectors).


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Lentigen has developed a novel highly efficient Lentiviral vector system LentiMax™. LentiMax is a highly optimized modular HIV-1 based Lentiviral vector that can be customized to accommodate any desired gene, gene silencing, promoter, post-transcription or insulator element. LentiMax can accommodate both self-inactivating (SIN) and non-SIN vector formats.

Innovations in Pharmaceutical Technology [pdf]
 
 
 
 
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