LentiMax™ delivers genes into a wide range of cell types stably with high efficiency.

The cell types shown were mixed with LentiMax™ expressing a green fluorescent reporter gene and analyzed by fluorescent photomiscroscopy and fluorescent activated cell sorting analysis (FACS).

While Lentigen's vectors are suitable for the transduction of many mammalian cell types, transduction efficiency is dependant on cell type, species of origin and different conditions, including the multiplicity-of-infection (MOI) used to treat the cells and exposure time to the vector. In some cases, the use of cationic compounds like Polybrene or DEAE-Dextran may be required to increase transduction efficiency.

To determine the most effective vector MOI for your target cells, perform a series of transductions with different concentrations of vector containing a GFP reporter (such as LentiGFP cat# LV-173GFP or LV-175GFP). Note that some cell types, such as primary cells and some suspension cultures may be more difficult to transduce and need additional treatments. Mouse cells (primary and cultured) may require as much as 10x higher MOI than human cells for efficient transduction.